Association Study of KCNJ11 and TCF7L2 Genetic Variants with Type 2 Diabetes Mellitus in Southern Punjab

Shaheen S, Aslam S, Shaheen M, Iqbal RK, Awan A and Jamil H

Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, Pakistan
Department of Forestry and Range Management, University of Agriculture, Pakistan

^*Correspondance to: Sabahat Shaheen 

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Abstract:

Diabetes is the most chronic disease that is caused due to genetic and environmental factors. The common genetic factor that is associated with T2D is single nucleotide polymorphism. The aim of the present study was to assess the association between rs5219 of the KCNJ11 gene and rs11196205 of the TCF7L2 gene with diabetes type 2. Total of 300 samples (150 T2DM patients and 150 nondiabetic controls) together with demographic factors were taken from public and private hospitals of the Southern Punjab. This was a case-control study and contained 150 T2D patients and 150 controlled samples. To analyze the polymorphisms in KCNJ11 and TCF7L2, PCR-RFLP and Tetra-arm PCR were used. The findings of this study were that in KCNJ11 gene, homozygous AA genotype was predominant (83.33%) than the GG genotype (14%) and heterozygous genotype GA was present in very a small amount (2.66%) while in case of TCF7L2 gene, CC 28.00%, GG 27.33% and GC 44.67%. The insignificant statistical association was found between rs5219, rs11196205 and T2D as p-value of KCNJ11 (rs5219) is 0.4125 and TCF7L2 (rs11196205) is 0.6. The conclusion of this study is that rs5219 of the KCNJ11 gene and rs11196205 of TCF7L2 gene are not associated with diabetes type 2 in the Southern Punjab population.

Keywords:

KCNJ11; TCF7L2; PCR-RFLP; Tetra-arm PCR; Insignificant

Cite the Article:

Shaheen S, Aslam S, Shaheen M, Iqbal RK, Awan A, Jamil H. Association Study of KCNJ11 and TCF7L2 Genetic Variants with Type 2 Diabetes Mellitus in Southern Punjab. Clin Case Rep Int. 2023; 7: 1555.