Protective Effects of Methanolic Extract of Telosma cordata against Aceclofenac Induced Hepatotoxicity in Rats

Fatima M and Rashid Khan M

Department of Biochemistry, Quaid-i-Azam University, Pakistan

^*Correspondance to: Mehreen Fatima 

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Abstract:

Telosma cordata is traditionally used as an effective agent for treating conjunctivitis and back pain but has not been thoroughly studied. In current study, the hepatoprotective aptitude of T. cordata was evaluated against Aceclofenac (ACF) instigated oxidative stress in rats. Leaves of T. cordata were extracted in methanol and various fractions were prepared by polarity-based method. Qualitative and quantitative spectrophotometric analysis along with HPLC-DAD analysis was performed on samples. Antioxidant potential of extracts was identified by DPPH method while BSA method was used to assess the anti-inflammatory activity. Rats were divided into various groups and oxidative stress was induced by ACF in liver of rats. T. cordata Methanol extract (TCM) was used to mitigate the oxidative stress. Serum analysis for liver enzymes and tissue analysis for enzymatic and nonenzymatic antioxidants was performed. Qualitative and quantitative spectrophotometric analysis of various extracts depicted the presence of phenolics, flavonoids, and tannins. In vitro antioxidant and anti-inflammatory studies further elaborated the protective potential of plant extracts. Furthermore, treatment of rats with ACF showed decline (p<0.05) in the level of liver antioxidant enzymes, in contrast, elevation was found in the concentration of hydrogen peroxide and nitrite in hepatic tissues. Increased serum markers of the liver were revealed after ACF treatment. Hepatocyte inflammation and injuries were prominent in histopathological studies of the liver in ACF-treated rats. However, the administration of TCM to ACF-intoxicated rats reduced liver injuries and restored the levels of antioxidant enzymes and free radical towards normal rats. T. cordata possess strong antioxidant and anti-inflammatory potential due to presence of polyphenols and can help in development of drug against liver injuries with strong antioxidant potential.

Keywords:

Telosma cordata; Antioxidants; Anti-inflammatory; Oxidative stress; Aceclofenac

Cite the Article:

Fatima M, Rashid Khan M. Protective Effects of Methanolic Extract of Telosma cordata against Aceclofenac Induced Hepatotoxicity in Rats. Clin Case Rep Int. 2023; 7: 1564.